In vitro activity of Allium sativum and Aloe vera extract against Salmonella enterica subspecies enterica serovar Gallinarum
DOI:
https://doi.org/10.61538/huria.v22i0.65Abstract
The rapid increase of antibiotic resistance needs to be taken as a threat to both animals and human being. In this study the arbitrary concentration of 25%, 50% and 75% of individual and combined Allium sativum and Aloe vera were tested against Salmonella Gallinarum. The antimicrobials were extracted using aqueous solution where 25g, 50g and 75g of Allium sativum were dissolved in 100ml of sterile distilled water and left for 24hrs and filtered to obtain the concentration of 25%, 50% and 75% respectively and 1ml, 2ml and 3ml of Aloe vera exudates were homogenized in 4ml of distilled water separately by centrifugation to obtain concentration of 25%, 50% and 75% respectively. Aqueous extract of Allium sativum and Aloe vera exudates of 25%, 50%, 75% concentration and 25ml, 50ml, 75ml respectively were homogenized together to obtain a mixture. The different concentration of single and mixtures were tested against Salmonella Gallinarum for growth inhibition through pour plate method. The concentration of 50% and 75% illustrated strong antimicrobial activities but Allium sativum was more effective than Aloe vera. Combined concentration of 25% was less effective but those of 50% and 75% were highly effective than corresponding concentration of single. The combined concentration at 50% and 75% could be used to treat or to control clinical cases in poultry production caused by Salmonella Gallinarum with low cost compared to the synthetic drugs which have become less effective and have high cost. Aloe vera and Allium sativum can be refined and processed in the pharmaceutical industries at required concentrations and sold to the farmers at low cost for inhibition and control of Salmonella Gallinarum in poultry production.  ÂReferences
Adeshina, G.O., Jibo, S., Agu, V.E. and Ehinmidu, J.O. (2011). Antimicrobial activity of fresh juice of Allium cepa and Zingiber officinale against multidrug resistant bacteria. International journal of pharmacology and biological science. 2: 289-295.
Adetumbi, M.A. and Lau, B.H. (1983). Allium sativum (garlic)–a natural antibiotic. Medical Hypothesis 12(3): 227-237.
Arunkumar, S. and Muthuselvam, M. (2009). Analysis of Phytochemicals Constituents and antimicrobial activities of Aloe vera L. against Clinical Pathogens. World Journal of Agricultural Sciences. 5 (5): 572-576.
Banna, A.H., Latif, A. and Soliman, M. (2013). Anticoccidial Activity of Allium Sativum and Aloe Vera in Broilers. International Journal for Agro veterinary and Medical Sciences. (7):117-125.
Belguith, H., Kthiri, F., Sofah, A.A., Hamida, B.J. and Landoulsi, A. (2010). Study of the effect of aqueous garlic extract (Allium sativum) on salmonella serovars isolates. Emirate journal of food and Agriculture. 22(3): 189-206.
Boudreau, M.D. and Beland, F.A. (2006). An evaluation of the biological and toxicological properties of Aloe barbadensis (miller), Aloe vera. Journal of Environmental Science and Health Part C. 24:103–154.
Durairaj, S., Srinivasan S., and Lakshmanaperumalsamy, P. (2009). In vitro antibacterial activity and stability of garlic extract at different pH and temperature. Electronic journal of Biology. 5(1): 5-10.
Erdogrul, O.T. (2002). Antimicrobial activities of some plant extracts used in fork medicine. Journal of Pharmaceutical biology. 40:269-273.
Iwalokun, B.A., Ogunledum, A., Ogbolu, D.O., Bamiro, S.B. and Omojola, J.J. (2004). In vitro antimicrobial properties of aqueous garlic extract against multidrug bacteria and Candida. Journal of medicinal food. 7(22), 327-333.
Kwon, Y.B., Hong, K.M., Hwang, Y.S., Shin, S., Back, H.J., and Park, H. Y. ( 2011). Antimicrobial and immunomodulatory effects of Aloe vera peel extract. Journal ofmedicinal plants research. 5 (22), 5384-5393.
Mikail, H. G. (2010). Phytochemicals screening, elemental analysis and acute toxicity of aqueous extract of Allium sativum L. bulbs in experimental rabbits. Journal of Medicinal Plants Research. 4(4), pp. 322-326.
Ogunjobi, A.A., Fagade, O.O., and David, O.O. (2007). Ant-mutagenic and potential anti-carcinogenic activities of Aloe vera gel and aqueous garlic extract in bacterial reverse mutation test. African Journal of Biomedical Research. (10): 275-278.
Reynolds T. and Dweck A.C. (1999). Aloe vera leaf gel: a review update. Journal of Ethnopharmacology. 68:3–37.
Sagdic, O. (2003). Sensitivity of four pathogenic bacteria to Turkish thyme and wild marjoram hydrosol. Swiss Journal of food science and technology. 36:467-473.
Vuorela, P., Leinonenb, M., Saikkuc, P., Tammelaa, P., Raushad, J.P., Wennberge, T. and Vuorela, H. (2004). Natural products in the processing of finding new drugs candidates. Journal of current medical chemistry. 11(11), 1375-1389.
Waihenya, R.K., Mtambo, M.M., Nkwengulila, G. and Minga, U.M. (2002). Efficacy of crude extract of Aloe secundiflora against Salmonella gallinarum in experimentally infected free-range chickens in Tanzania. Journal of Ethnopharmacology. 79:317–323.
Yabaya, A., Orukotan, A. and Jonathan, M. (2010). Determination of antiSalmonella typhi activity of crude extract of Allium sativum (garlic). Journal of biological science and biological conservation. 2: 22-18
Zalalem, A., Kabede, N., Sisay, S., Alemayehu, H., and Wubetie, T.K. (2011). Prevalence and antimicrobial resistance of Salmonella isolated from lactating cow. BMC infectious diseases. 11: 222.
